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validation rules is applicable to all page layout of a standard object but i want it has to apply for a particular page layout not other page Layout. Could any one help me?

Hi Santosh,

Actually Validation rules are record specific and not page layout specific  

If you have validation for e.g. a field has to be required when a record is entered - this is something that can be managed using the required validation controlled by page layout but for other validation rules you will have to probably create a visualforce(VF) page rather than a pagelayout and then apply custom validations on the VF page as shown in the following example: